Kinetic Analyses of Calcium Movements in HeLa Cell Cultures
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منابع مشابه
Kinetic Analyses of Calcium Movements in HeLa Cell Cultures
Calcium efflux was studied in monolayers of HeLa cells. The fast phase of exchange was studied in an open system by continuous washout. Its half-time was 1.58 min which is practically identical to the fast phase of calcium influx previously found to be 1.54 min. This suggests that the fast component of efflux represents calcium exchange from an extracellular compartment probably from calcium bo...
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Calcium influx was studied in monolayerg of HeLa cells to determine the number of exchangeable and nonexchangeable pools and the rate constant of the different fluxes. Of the two exchangeable pools, one has a very fast rate of exchange with a half-time of 1.54 illin, a compartment size of 1.06 m#moles/mg cell protein, and an exchange rate of 474 #/~moles/(mg protein. rain). This compartment is ...
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Calcium compartments and fluxes were measured by kinetic analyses in kidney cell suspensions in a three-compartment closed system. The fast phase influx and compartment size increase linearly with the medium calcium and the half-time of exchange is only 1.3 min which suggests that the fast component is extracellular. The slow phase compartment rises linearly from 0.1 to 0.5 mmole calcium/kg cel...
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HeLa cells in culture synthesize metallothionein. To investigate the effects of glucocorticoids on metallothionein synthesis we adapted HeLa cells to growth in a defined medium lacking hydrocortisone. In this defined medium, containing 1.5 x 10(-6)M Zn2+, dexamethasone (10(-6)M) caused a five- to sixfold increase in the synthesis of metallothionein. Progesterone (10(-5)M), a known antagonist of...
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 1969
ISSN: 1540-7748,0022-1295
DOI: 10.1085/jgp.53.1.43